甜瓜叶片高效再生体系的建立

为了建立高效稳定的甜瓜植株再生体系,为甜瓜的转基因技术提供受体材料,以薄皮甜瓜品种"西甜一号"叶片为外植体,通过器官发生途径诱导形成不定芽,研究了不同的激素组合、外植体放置方式、暗培养时间等条件对其再生的影响。结果表明,以新梢顶端展开的第2~3片叶在MS 6-BA1.0 mg/L的诱导培养基上,外植体远轴面向下接触培养基,直接转入正常光周期下培养再生频率可达100%,平均每个外植体上可诱导出11株健壮植株。在MS 6-BA 0.05 mg/L不定芽伸长培养基上,分化的不定芽(丛)能够伸长。在1/2MS IAA 0.2 mg/L的生根培养基上无根苗生根,生根率可达100%。

High Frequency Shoot Regeneration System from Leaf Explants of Melon

In order to establish an efficient regeneration system via organogenesis in thin-skin melon(Cucumis melo L.) and offer a kind of reference materials for genetic transformation of melon,the leaf explants were used and some factors included concentration of plant growth regulators,orientation of the leaf and period of dark culture were studied.The results showed that the best medium for shoot regeneration was MS added 1.0 mg/L 6-BA.When the explants were cultured in normal photoperiod,no dark treatment,with abaxial side touching the optimal medium,the buds regeneration rate was 100% with 11 shoots per leaf disc.The concentration of plant growth regulators was 0.05 mg/L6-BA during the stage of bud stretching.On the medium 1/2 MS IAA 0.2 mg/L,the elongated shoots rooted at the rate of 100%.