| 猪伪狂病病毒粤A株gE基因的克隆与序列分析 |
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| 引用本文： | 耿忠海,贺东生,李健,罗满林,黄毓茂,刘镇明,黄桂菊.猪伪狂病病毒粤A株gE基因的克隆与序列分析[J].中国预防兽医学报,2005,27(5):352-355. |
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| 作者姓名： | 耿忠海 贺东生 李健 罗满林 黄毓茂 刘镇明 黄桂菊 |
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| 作者单位： | 1. 华南农业大学,广东,广州,510642
2. 上海检验检疫局,上海,200000 |
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| 摘 要： | 参考GenBank中伪狂犬病病毒(PRV)gE基因的序列设计了1对引物,对PRV粤A株gE基因进行了PCR扩增,PCR产物克隆到PMD18-T载体.对重组质粒进行限制性内切酶分析和基因测序,证实了克隆片段的可靠性,并与2株不同来源的毒株进行了同源性分析和比较.
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| 关 键 词： | 伪狂犬病病毒 gE基因 克隆 序列分析 |
| 文章编号： | 1008-0589(2005)05-0352-04 |
| 收稿时间： | 2004-06-03 |
| 修稿时间： | 2004年6月3日 |
Cloning and sequence analysis of the gE gene of pseudorabies virus Yue A strain |
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| GENG Zhong-hai,HE Dong-sheng,LI Jian,LUO Man-lin,HUANG Yu-mao,LIU Zhen-ming,HUANG Gui-ju.Cloning and sequence analysis of the gE gene of pseudorabies virus Yue A strain[J].Chinese Journal of Preventive Veterinary Medicine,2005,27(5):352-355. |
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| Authors: | GENG Zhong-hai HE Dong-sheng LI Jian LUO Man-lin HUANG Yu-mao LIU Zhen-ming HUANG Gui-ju |
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| Abstract: | A pair of primers were designed according to the sequences published in the GenBank in order to amplify the gE gene of the pseudorabies virus YA strain.The gE gene was isolated by polymerase chain reaction,and subsequencely cloned into the PDM-(18)-T vector.The recombinant plasmid was identified by restriction enzyme analysis and nucleotide sequencing,which completely proved its validity.The homology of the gE gene of PRV YA strain with two different strains were studied. |
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| Keywords: | pseudorabies virus gE gene cloning nucleotide sequencing |
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