五指山小型猪近交系白细胞抗原Ⅰ类3 基因的研究

 【目的】为探讨五指山小型猪（WZSP）近交系群体中SLAⅠ3 (SLA-3) 位点等位基因分布结构及其特性。【方法】利用4对引物，采用RT-PCR扩增了32头WZSP SLA-3基因部分外显子1、外显子2和大部分外显子3序列。根据家系和扩增结果，选择8头个体的PCR产物克隆测序。【结果】获得9个不同的核苷酸序列。与GenBank中所用SLA-3基因的核苷酸序列及其编码的氨基酸序列进行比较分析，确定这9个序列均为SLA-3位点上的新等位基因，但这些等位基因间核苷酸变异很少；氨基酸序列比较发现猪和人之间有很高的保守性。同时，构建了23个SLA-3的等位基因和1个HLA-A等位基因核苷酸序列的系统树。【结论】WZSP在SLA-3位点和其它猪品种有明显的差异，拥有其独特的遗传资源。从分子水平为WZSP SLA-3基因的分子分型及特异单倍体猪的培育和异种器官移植实验用动物的研究提供理论依据。

Study on SLA Class Ⅰ-3 Gene for Inbreeding WZSP

To elucidate the structure of SLAⅠ3 alleles on inbred line of Wuzhishan pig（WZSP） population. RT-PCR products of the partial exon1, completed exon2 and partial exon3 of the SLA classical I class 3 gene were successed to amplify in 32 WZSPs. According to genealogy and amplification results, PCR products of 16 WZSPs were selected to clone and sequence. 10 different nucleotide sequences were obtained. The sequences data and their coden amino acids sequences were compared with all sequences of SLAⅠ3 gene in GenBank, respectively. It was found that these sequences were different in comparison with each other or with those of published sequences of SLAⅠ3 gene, but there were very few variation among them. The data of comparing the amino acids sequences indicated that most of these sites are conserved among the related molecules between porcine and human. Meanwhile, construction of a phylogenetic tree using the nucleotide sequences of those 23 alleles and 1 HLA A gene indicated that WZSP population own its unique resource.