犬细小病毒的分离鉴定及其VP2基因序列分析

本试验从北京某动物医院疑似犬细小病毒(CPV)感染犬粪便中分离出一株病毒。通过电子显微镜观察、血凝试验、动物回归试验和分子生物学鉴定,本试验成功分离出一株CPV,命名为BJ-24。对BJ-24株的VP2基因序列分析发现该分离株为CPV-2a亚型,其VP2基因与GenBank中19株CPV VP2基因核苷酸序列有较高的同源性,均在98.7%以上,甚至与CPV-JS2株达到100.0%。系统进化分析结果表明所分离的BJ-24株属于中国分支,与CPV-JS2株遗传距离最近。本试验对进一步开展北京地区CPV的流行病学调查提供基础依据,并对防制病毒传播和疫苗研制奠定了坚实的基础。

Isolation and Identification of Canine Parvovirus Virus and Sequence Analysis of VP2 Gene

A strain of virus was isolated and identified from feces of a suspected canine parvovirus (CPV) infected dog.It was eventually identified as CPV using electron microscope examination,hemagglutination (HA) test,animal regression experiment and molecular biology idenfication,designated as BJ-24 strain.The VP2 gene sequence analysis of BJ-24 strain showed that BJ-24 strain belonged to CPV-2a subtype.The analysis of VP2 gene indicated that the BJ-24 VP2 gene shared higher homology of nucleotide with 19 CPV strains available in GenBank and were more than 98.7%.The highest homology was 100.0% with CPV-JS2 strain.Phylogenetic tree analysis showed that BJ-24 strain belonged to China cluster and there was the closest distant between BJ-24 strain and CPV-JS2 strain.This study provided the basic evidence for further study on molecular epidemiology in Beijing and laid the solid foundation for developing vaccine and preventing the spread of CPV.