AQP7基因内含子4多态性与郏县红牛生长性状的关联分析

本试验旨在研究郏县红牛AQP7基因内含子4的遗传变异,分析其遗传多态性对生长性状的影响。采用PCR-SSCP、DNA序列分析及生物信息学技术探讨郏县红牛AQP7基因内含子4的多态性及其与生长性状的关联情况。结果发现,郏县红牛AQP7基因内含子4存在A、B、C 3个等位基因,其基因频率依次为0.838、0.076、0.086;郏县红牛AQP7基因4种不同基因型与其体重和胸围有显著相关(P< 0.05),与其他指标不存在相关性(P> 0.05),且BC基因型个体的体重和胸围均显著大于其他3种基因型(P< 0.05)。暗示BC基因型是提高郏县红牛生长性状的有利基因型,AQP7基因内含子4的遗传多态性与郏县红牛的生长性状存在相关,因此可作为该品种生长性状分子标记辅助选择的候选基因。

Association Analysis on Polymorphisms of AQP7 Gene Intron 4 with Growth Traits in Jiaxian Cattle

The objectives were to research the genetic variations in intron 4 of AQP7 gene in Jiaxian cattle, and analyze the influence of genetic polymorphisms on growth traits.PCR-SSCP technique, DNA sequence analysis and bioinformatics were used to analyze the association between the polymorphisms of the intron 4 of AQP7 gene and growth traits in Jiaxian cattle.The results showed that there were three alleles A, B, C in this locus with the corresponding frequencies of 0.838, 0.076 and 0.086;The polymorphisms of the intron 4 of AQP7 gene had significant association with body weight and chest circumference (P< 0.05), while had no significant association with other growth traits (P> 0.05).The individuals with BC genotype was significantly higher than those of individuals with other genotypes in body weight and chest circumference (P< 0.05).It suggested that BC genotype had positive effect on growth traits, and the correlation was detected between the polymorphisms of the intron 4 of AQP7 gene and growth traits in Jiaxian cattle.So AQP7 gene could be used as a candidate gene for molecular marker assisted selection of growth traits in Jiaxian cattle.