| 应用RAPD方法获得与番茄ToMV抗性基因Tm2nv连锁的分子标记 |
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| 引用本文： | 田苗英,冯兰香,杨翠荣.应用RAPD方法获得与番茄ToMV抗性基因Tm2nv连锁的分子标记[J].植物病理学报,2000,30(2). |
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| 作者姓名： | 田苗英 冯兰香 杨翠荣 |
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| 作者单位： | 中国农业科学院蔬菜花卉研究所!北京100081 |
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| 基金项目： | ARC- AVRDC，植物病虫害生物学国家重点实验室开放基金资助项目 |
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| 摘 要： | 运用 RAPD技术 ,在番茄 To MV抗性基因 Tm2 nv的 F2 代群体中采用混合分组分析法 ( bulkedsegregant analysis,BSA)进行分子标记研究 ,找到了一个与 Tm2 nv基因连锁的分子标记 OPD2 0 170 0 ,其遗传距离为 7.0 67c M,L OD值为 16.768
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| 关 键 词： | 番茄 番茄花叶病毒 随机扩增多态性DNA 分子标记 |
IDENTIFICATION OF A MOLECULAR MARKER LINKED TO ToMV RESISTANCE GENE Tm2~(nv) IN TOMATO USING RANDOMLY AMPLIFIED POLYMORPHIC DNA |
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| TIAN Miao-ying,FENG Lan-xiang,YANG Cui-rong,GONG Hui-zhi.IDENTIFICATION OF A MOLECULAR MARKER LINKED TO ToMV RESISTANCE GENE Tm2~(nv) IN TOMATO USING RANDOMLY AMPLIFIED POLYMORPHIC DNA[J].Acta Phytopathologica Sinica,2000,30(2). |
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| Authors: | TIAN Miao-ying FENG Lan-xiang YANG Cui-rong GONG Hui-zhi |
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| Abstract: | Randomly amplified polymorphic DNA (RAPD) was employed as a tool to detect molecular markers linked to the ToMV resistance gene Tm2 nv in tomato. A specific DNA band OPD20 1700 was proved to be linked to the gene Tm2 nv by bulked segregating analysis (BSA) with F 2 population. The genetic distance was measured as 7.067cM and LOD was 16.768. |
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| Keywords: | tomato ToMV RAPD molecular marker |
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