长寿花离体快繁研究

目的]研究长寿花离体快繁的培养基配方和培养条件。方法]以长寿花的幼嫩叶片作为外植体,以MS为基本培养基,诱导出不定芽进行快速繁殖,并比较添加4种浓度的BA和NAA对长寿花不定芽的诱导和生根效果的影响。结果]长寿花幼嫩叶片的诱导和生长与生长素、细胞分裂素2种激素有关系,两者的配比直接影响了叶片的再生频率。长寿花叶片诱导和不定芽增殖的最适培养基为MS+6-BA1.0 mg/L+NAA0.5 mg/L,最适生根培养基为1/2 MS+NAA0.5 mg/L,不定芽在此培养基上的生根率最高,移栽成活率为95%。通过直接诱导可获得较高的不定芽诱导率,同时增殖培养的增殖系数达到10,大大缩短了常规育苗时间。结论]该研究建立了长寿花组织培养的再生体系,为其快速繁殖及大规模的工厂化育苗提供科学依据。

Study on in Vitro Rapid Propagation of Kalanchoe blossfeldiana

Objective] The aim of the research was to study the medium formula and culture conditions for in vitro rapid propagation of Kalanchoe blossfeldiana.Method] With tender leaves of K.blossfeldiana as explant and MS as basic medium,adventitious buds were induced for rapid propagation.And the effects of adding 4 concentrations of BA and NAA on the induction of adventitious buds and rooting effect of K.blossfeldiana were compared.Result] The induction and growth of tender leaves in K.blossfeldiana had relations with 2 kinds of hormone including auxin and cytokinin,and the matching of auxin and cytokinin had a direct effect on the regeneration frequency of leaves.The optimum medium for the induction of leaves and the proliferation of adventitious buds was MS 6-BA1.0 mg/L NAA 0.5 mg/L and the optimum rooting medium was 1/2 MS NAA 0.5 mg/L on which the adventitious buds obtained highest root rate with their transplanting surviving rate of 95 %.By direct induction,higher inducement rate of adventitious buds could be obtained and propagation coefficient of propagation culture reached 10,which shortened the time of conventional seedling.Conclusion] The research established the regeneration system of tissue culture in K.blossfeldiana and provided scientific basis for its rapid propagation and factory-cultivated seedling in large scale.