Detection of intra- and interspecific variation of the dry rot fungus <Emphasis Type="Italic">Serpula lacrymans</Emphasis> by PCR-RFLP and RAPD analysis |
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| Authors: | Email author" target="_blank">Sakae?HorisawaEmail author Yoh?Sakuma Katsuhiko?Takata Shuichi?Doi |
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| Institution: | (1) Institute of Wood Technology, Akita Prefectural University, Noshiro 016-0876, Japan;(2) Biological Resources Division, Japan International Research Center for Agricultural Sciences, Tsukuba 305-0851, Japan;(3) Present address: Department of Environmental Systems Engineering, Faculty of Engineering, Kochi University of Technology, Kami-gun, Kochi 782-8502, Japan |
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| Abstract: | We investigated a genotype-based assay to discriminate the dry rot fungi Serpula lacrymans. DNAs were extracted from 74 isolates from the northern half of Japan, and internal transcribed spacers (ITS) were amplified by polymerase chain reaction. Genotypes of isolates were checked by restriction fragment length polymorphism (RFLP) using two enzymes, Taq I and Hha I. Among the 74 isolates identified as S. lacrymans in terms of morphologic features, 5 isolates were shown to have been misidentified. Random amplified polymorphic DNA (RAPD) analysis was conducted in order to detect the intraspecific diversity of S. lacrymans isolated in Japan. Because no relation between geographical origin and genetic distances was observed, the intraspecific diversity of S. lacrymans is suggested to be small.Part of this report was presented at the 52nd Annual Meeting of the Japan Wood Research Society, Gifu, April 2002 |
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| Keywords: | Serpula lacrymans PCR-RFLP ITS RAPD |
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