| 以淀粉酶为标记选择水稻白叶枯病菌毒性基因突变体 |
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| 引用本文： | 何晨阳 董晓艳. 以淀粉酶为标记选择水稻白叶枯病菌毒性基因突变体[J]. 南京农业大学学报, 1992, 15(3): 30-34 |
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| 作者姓名： | 何晨阳 董晓艳 |
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| 作者单位： | 植物保护学系 210014(何晨阳，董晓艳，刘文华)，植物保护学系 210014(王金生) |
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| 基金项目： | 国家高技术发展计划863项目资助课题 |
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| 摘 要： |
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| 关 键 词： | 淀粉酶 水稻 白叶枯病 毒性基因 |
TO ISOLATE VIRULENCE GENE MUTANTES OF XANTHOMONAS CAMPESTRISPV.ORYZAEUSING AMYLASE ACTVITY AS A MARKER |
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| He Chenyang,Dong Xiaoyan,Liu Wenhua,Wang Jinsheng. TO ISOLATE VIRULENCE GENE MUTANTES OF XANTHOMONAS CAMPESTRISPV.ORYZAEUSING AMYLASE ACTVITY AS A MARKER[J]. Journal of Nanjing Agricultural University, 1992, 15(3): 30-34 |
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| Authors: | He Chenyang Dong Xiaoyan Liu Wenhua Wang Jinsheng |
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| Abstract: | Transposon Tn5 mutagenesis in a wildtype strain JXO I of Xanthomonas campestris pv.oryzaewas conducted.Screening of 6 250 Tn5-induced clones on plates using amylase activity as a marker led to theisolation of 14 virulence gene mutants avirulent on susceptible rice cultivar IR26,4 of which lost the abilityto elicit a hypersensitive response on tobacco plants and were identified as hrp gene mutants.The pleiotropicalternation of phenotype of these mutants included the growth characteristics,and the activity ofprotenase,petase,cellulase and lipolysase.Southern blot analysis of EcoRI digested total DNA from allavirulent mutants with ~(32)p labelled Tn5-containing plasmid revealed that insertion of Tn5 occurred in at lesat4 different sites. |
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| Keywords: | Xanthomonas campestris pv.oryzae Transposon(Tn5)mutagenesis amylase activity virulence gene mutants |
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