| 八角基因组DNA的提取方法 |
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| 引用本文： | 陈海云,吴涛,耿树香,白平,陈少瑜,宁德鲁.八角基因组DNA的提取方法[J].福建林业科技,2012,39(4):26-29. |
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| 作者姓名： | 陈海云 吴涛 耿树香 白平 陈少瑜 宁德鲁 |
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| 作者单位： | 1. 云南省林业科学院,云南昆明,650204
2. 云南省林业科学院,云南昆明650204;云南省森林植物培育与开发利用重点实验室/国家林业局云南珍稀濒特森林植物保护和繁育重点实验室,云南昆明650204 |
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| 基金项目： | 云南省自然科学基金(2010ZC234);云南省重点新产品开发计划项目(2009BB006)资助 |
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| 摘 要： | 采用4种植物基因组DNA提取方法(CTAB法、SDS法、高盐法、试剂盒法)对八角基因组DNA进行提取试验.结果表明,对传统CTAB法稍加改良可以得到较为理想的提取效果,改良的内容包括①在加入CTAB提取缓冲液之前,先用CTAB-free缓冲液抽提1～2次；②将CTAB提取缓冲液中CTAB的用量从2％提高到3％；③提取缓冲液的用量由样品干重的10倍增加到60倍.用改良CTAB法提取的DNA经RAPD-PCR扩增,条带清晰,其质量能够满足分子标记的要求.
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| 关 键 词： | 八角 基因组DNA 改良CTAB法 凝胶电泳 RAPD-PCR扩增 |
Methods for Genomic DNA Extraction from Illicium verum |
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| CHEN Hai-yun,WU Tao,GENG Shu-xiang,BAI Ping,CHEN Shao-yu,NING De-lu.Methods for Genomic DNA Extraction from Illicium verum[J].Journal of Fujian Forestry Science and Technology,2012,39(4):26-29. |
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| Authors: | CHEN Hai-yun WU Tao GENG Shu-xiang BAI Ping CHEN Shao-yu NING De-lu |
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| Institution: | 1(1.Yunnan Academy of Forestry,Kunming 650204,Yunnan,China; 2.Key Laboratory of Forestry Plant Cultivation and Utilization of Yunnan Province,Yunnan Laboratory for Conservation of Rare,Endangered & Endemic Forest Plants,State Forestry Administration,Kunming 650204,Yunnan,China) |
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| Abstract: | The genomic DNA of Illicium verum were extracted with 4 plant genomic DNA extraction methods,namely CTAB,SDS,high concentration salt and reagent kit.The results showed that the improved CTAB could achieve promising extraction effect.The modification included using CTAB-free buffer to wash out for 1～2 times prior to the procedure of adding CTAB extraction buffer;adjusting the used content of CTAB in extraction buffer from 2 % to 3 %;adding the extraction buffer to 60 times of the dry weight of leaf sample from 10 times.Using the DNA extracted by the improved CTAB method,through RAPD-PCR amplification,clear bands were achieved;and the quality of extracted DNA could sufficiently meet the requirements of further molecular marker experiment. |
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| Keywords: | Illicium verum genomic DNA improved CTAB method gel electrophoresis RAPD-PCR amplification |
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