N-乙酰半胱氨酸联合家蚕丝素蛋白解酒效果的动物试验

为了开发解酒效果更好的蚕丝功能性食品,将具有氧自由基清除作用,易进入肝细胞的N-乙酰半胱氨酸添加入家蚕丝蛋白粉中,调查其对ICR种小鼠血液中乙醇的分解效果。N-乙酰半胱氨酸联合家蚕丝素蛋白灌胃处理较家蚕丝素蛋白灌胃处理对小鼠血液中的乙醇具有更好的分解效果:前者使小鼠血液中的乙醇浓度在灌胃乙醇30 min后就快速下降,至4 h时,小鼠血液中的乙醇浓度仅为对照组的8.1%;后者使小鼠血液中的乙醇浓度在灌胃乙醇120 min后开始下降,至4 h时,小鼠血液中的乙醇浓度为对照组的65.6%。N-乙酰半胱氨酸联合丝素蛋白对雌、雄ICR种小鼠和SD种大鼠的急性毒性试验结果显示,其LD50均大于10 g/kg,且在试验剂量范围(2.5、5.0、10.0 g/kg)内,对小鼠骨髓多染红细胞微核率及精子畸形发生率均未见有明显影响。在丝素蛋白粉中添加适量N-乙酰半胱氨酸后,能增强对供试动物血液中乙醇的分解作用,且无明显的毒副作用。

Animal Trials for the Anti-alcoholic Effect of N-acetylcysteine Combined Silk Fibroin

In order to develop functional foods with good anti-alcoholic effect from silk fibroin,the present study investigated the alcohol degradation effect of silk fibroin powder supplemented with N-acetylcysteine which has good free radical scavenging effect and easy access into liver cells in ICR mice.The results showed that N-acetylcysteine combined silk fibroin had better alcohol degradation effect in mouse blood than silk fibroin alone.The former brought about a quick reduction of the mouse blood alcohol leve...