| 巨桉组织培养及工厂化育苗技术的研究 |
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| 引用本文： | 陈碧华,李乾振,吴丽君,翁秋媛,王志洁,陈达,洪志猛.巨桉组织培养及工厂化育苗技术的研究[J].福建林业科技,2006,33(1):61-63,79. |
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| 作者姓名： | 陈碧华 李乾振 吴丽君 翁秋媛 王志洁 陈达 洪志猛 |
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| 作者单位： | 福建省林业科学研究院,福建,福州,350012 |
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| 基金项目： | 福建省林业厅科研项目(闽林综[2003]126号) |
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| 摘 要： | 巨桉是造纸和人造板的良好材料,其组织培养微繁殖体系已经研究成功。取优树主干基部萌芽或扦插苗1~1.5 cm长的茎段做为外植体,外植体和增殖培养在附加有BAP和NAA的MW培养基上进行。培养条件为26±2℃,日光灯光照10 h.d-1。增殖培养采用BAP 0.1~0.5 mg.L-1和NAA 0.01~0.1 mg.L-1。离体的茎芽适合在B2附加0.5 mg.L-1IBA生根。小苗移栽红心土上,在简易温室成活率高达93%。
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| 关 键 词： | 巨桉 组织培养 离体 茎芽增殖 生根培养 |
| 文章编号： | 1002-7351（2006）01-0061-03 |
| 收稿时间： | 2005-07-26 |
| 修稿时间： | 2005-07-26 |
Studies on Tissue Culture and Commercial Production of Eucalyptus grandis |
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| CHEN Bi-hua,LI Qian-zhen,WU Li-jun,WENG Qiu-yuan,WANG Zhi-jie,CHEN Da,HONG Zhi-meng.Studies on Tissue Culture and Commercial Production of Eucalyptus grandis[J].Journal of Fujian Forestry Science and Technology,2006,33(1):61-63,79. |
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| Authors: | CHEN Bi-hua LI Qian-zhen WU Li-jun WENG Qiu-yuan WANG Zhi-jie CHEN Da HONG Zhi-meng |
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| Abstract: | A micropropagation system was developed for Eucalyptus grandis Hill ex Maiden,which is widely used for pulp and artificial boards.Shoots of 1～1.5 cm long from the base of plus tree trunks or cutting seedlings served as explants.The explants and shoot multiplication cultures were cultured on MW medium supplemented with combinations of BAP and NAA.Cultures were maintained at 26±2℃ and exposed to 10-h photoperiods of coolwhite fluorescent light.Shoot multiplication cultures were maintained by BAP at 0.1～0.5 mg·L~(-1) and combined with 0.01～0.1 mg·L~(-1) NAA.In-vitroproduced shoots were suitable to root on 0.5 mg·L~(-1) IBA supplemented to medium B2.Plantlets survived in red soil and exhibited high survivability(more than 93%) under simple greenhouse conditions. |
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| Keywords: | Eucalyptus grandis Hill ex Maiden tissue culture In vitro shoot multiplication rooting culture |
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