| 转几丁质酶和葡聚糖酶双价基因棉花株系对黄萎病的抗性 |
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| 引用本文： | 朱荷琴,冯自力,李志芳,赵丽红,师勇强,尹志新.转几丁质酶和葡聚糖酶双价基因棉花株系对黄萎病的抗性[J].棉花学报,2011,23(1):58-63. |
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| 作者姓名： | 朱荷琴 冯自力 李志芳 赵丽红 师勇强 尹志新 |
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| 作者单位： | 中国农业科学院棉花研究所/农业部棉花遗传改良重点实验室,河南,安阳,455000 |
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| 基金项目： | 转基因生物新品种培育重大专项课题(2009ZX08011-26B、2008ZX08011-002); 科技基础性工作专项(SB2007FY027); 国家公益性农业科研专项(3-19) |
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| 摘 要： | 采用人工病圃、重病田及苗期室内鉴定相结合的方法，研究了转双价（Chi+Glu）基因的棉花株系61217-1对棉花黄萎病的抗性。结果表明，转基因棉花株系61217-1在人工病圃连续3年达抗病水平，病指极显著低于其受体中棉所24。2009年该转基因株系在河南安阳、江苏大丰及新疆石河子棉花黄萎病重病田的病指分别为18.7、18.1和16.7，均极显著低于受体中棉所24的病指。该转基因株系对来自河北、湖北、新疆的3个强致病力落叶型菌株均达抗病水平，病指分别为16.4、16.8和15.6，也极显著低于受体中棉所24的病指。表明转双价（Chi+Glu）基因的棉花株系61217-1具有优异稳定的抗黄萎病性。
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| 关 键 词： | 转基因棉花 黄萎病 几丁质酶基因 葡聚糖酶基因 |
| 收稿时间： | 2010-07-30; |
Verticillium Wilt Resistance of A Transgenic Cotton Line with Chitinase and Glucanase Genes |
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| ZHU He-qin,FENG Zi-li,LI Zhi-fang,ZHAO Li-hong,SHI Yong-qiang,YIN Zhi-xin.Verticillium Wilt Resistance of A Transgenic Cotton Line with Chitinase and Glucanase Genes[J].Cotton Science,2011,23(1):58-63. |
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| Authors: | ZHU He-qin FENG Zi-li LI Zhi-fang ZHAO Li-hong SHI Yong-qiang YIN Zhi-xin |
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| Institution: | ZHU He-qin,FENG Zi-li,LI Zhi-fang,ZHAO Li-hong,SHI Yong-qiang,YIN Zhi-xin(Cotton Research Institute of CAAS/Key Laboratory of Cotton Genetics Improvement,MOA,Anyang,Henan 455000,China) |
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| Abstract: | Artificial disease nursery,severe disease field and greenhouse were jointly used for Verticillium wilt disease resistance identification of a transgenic cotton line 61217-1 into which chitinase and glucanase genes were transferred.Results showed that transgenic line 61217-1 exhibited high resistant level to Verticillium wilt disease in artificial nursery during successive three years.Average disease index of line 61217-1 was 17.9,which was significantly lower than that of the acceptor CCRI 24.In 2009,in the... |
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| Keywords: | transgenic cotton Verticillium wilt chitinase gene glucanase gene |
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