Expression Analysis of TGF-βRⅠ Gene in Ovary of AoHan Fine-wool Sheep at Different Tissues and Estrous Cycles

This study was aimed to investigate the expression of transforming growth factor-beta receptor Ⅰ(TGF-βR Ⅰ) in main tissue of AoHan Fine-wool sheep,the expression variations of ovary in different estrous states,and the mechanism of TGF-βRⅠ gene regulation in seasonal estrus of AoHan Fine-wool sheep. Twenty-four seasonal estrus AoHan Fine-wool sheep were chosen and divided into 4 groups of anestrus, proestrus, estrus and dioestrum with 6 sheep per group. First of all,the expression of TGF-βRⅠ gene in twelve tissues (thyroid,hypothalamus,hypophysis,ovary,adrenal gland,heart,liver,spleen,lung,kidney,pancreas and muscle) of AoHan Fine-wool sheep in the estrous stage and its expression variations in ovary of anestrus,proestrus,estrus and dioestrum were detected by Real-time PCR. The results showed that TGF-βRⅠ gene expressed in all the 12 tissues,and its expression in ovary and thyroid were extremely significantly higher than that in others (P<0.01). The expression of TGF-βRⅠ gene in hypothalamus,hypophysis,pancreas,adrenal gland, spleen and lung were high,which were significant higher than heart,liver,kidney and muscle (P<0.05).The expression levels of TGF-βRⅠgene in proestrus were extremely higher than other stages (P<0.01). The expression in dioestrum were the lowest which extremely lower than other stages (P<0.01). The above results indicated that TGF-βRⅠ might play an important role in turning on the maturation of follice.