Study on the Relationship between the Polymorphisms of PRLR Gene and Lactation Traits in Jiangyue Donkeys |
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Authors: | BI Lan-shu XIAO Hai-xia ZANG Chang-jiang HE Mei-sheng TUOHUTI Ajide PAREHATIJIANG Wufuer YU Xi LIU Wu-jun |
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Institution: | 1. College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China;
2. Institute of Livestocks, Xinjiang Academy of Animal Science, Urumqi 830000, China |
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Abstract: | In this study, the single nucleotide polymorphism of prolactin receptor (PRLR) gene and its correlation with the lactation traits in Jiangyue donkeys was tested, and the molecular genetic marker of breeding Jiangyue donkeys was explored.120 individuals were analyzed association of PRLR gene polymorphism with lactation traits in Jiangyue donkeys. Genetic polymorphism of PRLR gene flanking region was detected by PCR-SSCP method. The results showed that there was 1 mutation in PRLR gene and 2 genotypes of AA and AB, and allele A was the dominant allele. The SNP g.29764584 C>G was identified of PRLR gene by sequencing, and it was a synonymous mutation, did not cause amino acid changes. Statistical results indicated that the SNP was significantly associated with average daily milk yield and protein percentage in Jiangyue donkey (P<0.05),there was no significant in the lactin percentage(P>0.05). The mutation might be as crucial DNA genetic marker for lactation performance selection in Jiangyue donkeys. |
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Keywords: | Jiangyue donkeys PRLR gene polymorphism lactation traits |
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