Determine the Different Expression miRNAs in Different Months Old Xiang Pigs by miRNA Microarray |
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Authors: | LI Sheng XU Ya-nan NIU Xi RAN Xue-qin |
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Institution: | 1. Institute of Agro-bio Engineering, Guizhou University, Guiyang 550025, China;
2. Faculty of Animal Science, Guizhou University, Guiyang 550025, China |
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Abstract: | To determine the roles of miRNA in the developmental stages of Xiang pig,we investigated the miRNA expression patterns in different months old of Xiang pigs.The total RNA from 3 and 7 months old of Xiang pigs were collected separately. Then,the total RNA were hybridized with the miRNA microarray and the threshold (fold-change>2, P<0.05) were used to identify the different expression miRNA. The quantitative Real-time PCR was performed to verify the microarray result. Compared to the 3 months old group,there were 11 miRNA down regulated and 9 miRNA were up regulated in the 7 months old group. The lowest down regulated miRNA was miR-27b whereas the highest up regulated one was miR-155.The result of quantitative Real-time PCR consistented with the microarray result. The result suggested that some of the dysregulated expression of miRNA might participate in the lipid metabolism in hepatic cells. |
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Keywords: | miRNA expression profile Xiang pig liver |
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