苦马豆果实中总黄酮的提取和含量测定

[目的]探索苦马豆果实中总黄酮的提取方法和含量测定方法。[方法]以采集于青海省贵德县的苦马豆果实为材料，运用微波技术提取该果实中的黄酮类物质，并以芦丁为对照品，采用分光光度法测定提取物中的黄酮类物质的总含量。[结果]采用微波技术从苦马豆果实中提取出黄酮类物质，对其进行全波长扫描操作，样品在波长510nm处显示最大吸收，吸光度为0．242，计算出苦马豆果实中总黄酮含量为2．17％。5次平行测定的精密度相对偏差RSD为1．14％。样品液稳定性实验表明，吸光度值在5—45min内基本不变。回收率实验表明，测定平均回收率为98．86％，RSD为2．73％。[结论]采用微波技术可提高总黄酮的提取效率。采用分光光度法的测定方法简便、快速、灵敏，结果准确可靠，可作为苦马豆果实中总黄酮的含量测定方法。

Extraction of Total Flavonoids from Swainsonia salsula Fruit and Its Content Determination

[Objective] The research aimed to explore the methods of extraction of total flavonoids from Swainsonia salsula fruit and content determination.[Method] With S.salsula fruit gathered in Guide County,Qinhai Province as materials,the flavone substance was extracted from the fruits by using microwave technology,and with Rutin as control sample,the total content of flavone substance in extracts was determined with spectrophotometry.[Result] The flavone substance was extracted from S.salsula fruits by adopting m...