三种植物次生物质对B型烟粉虱羧酸酯酶活性及其对药剂敏感度的影响

采用人工饲料添加法,研究了不同浓度的槲皮素、2-十三烷酮和葫芦素B 3种植物次生物质对B型烟粉虱Bemisia tabaci羧酸酯酶(CarE)活性的影响,同时比较研究了用3种植物次生物质处理后敌敌畏、脱叶磷、灭多威、乐果和氧乐果5种药剂对CarE 的抑制中浓度(I50)的变化。结果表明: 0.01~1.0 mg/mL的槲皮素对B型烟粉虱成虫CarE活性均具有明显的诱导增加作用,最高为对照的4.32倍; 0.1和0.5 mg/mL的2-十三烷酮处理使CarE活性比对照分别下降了22%和58%; 0.75~30.0 mg/L的葫芦素B对烟粉虱CarE活性均表现为抑制作用;用1.0 mg/mL的槲皮素处理24 h后,敌敌畏和脱叶磷对烟粉虱CarE的I50值分别增加为对照的20.05和3.16倍; 3.75 mg/L 的葫芦素B处理后,敌敌畏和灭多威对CarE 的I50值分别增加为对照的25.94、2.45倍; 1.0 mg/mL的2-十三烷酮处理后敌敌畏、脱叶磷和乐果对CarE 的I50值分别降低了89%、83%和90%。

Effects of Three Allelochemcals on the Carboxylesterase Activity and itsSusceptibility to Insecticides in Bemisia tabaci Biotype B

The effects of quercetin,2-tridecanone and cucurbitacin B on the specific activity of carboxylesterase (CarE) in the tobacco whitefly,Bemisia tabaci (Gennadius) type B were investigated by feeding on artificial diet.The sensitivities of CarE to dichlorvos,methomyl,dimethoate,omethoate and S,S,S-tributyl phosphorotrithioate(DEF),an inhibitor of CarE,were also determined.After treated with quercetin ranged from 0.01 mg/mL to 1.0 mg/mL for 24 h,the specific activity of CarE increased obviously and the highest was 4.32-fold higher than the control. The specific activity of CarE was partially inhibited by 22% and 58%,respectively in the 0.1 and 0.5 mg/mL 2-tridecanone treatments. The cucurbitacin B ranged from 0.75 to 30.0 mg/L inhibited activity of CarE obviously.The I₅₀ values of dichlorvos and DEF to the CarE from B.tabaci treated with 1.0 mg/L quercetin were respectively 20.05- and 3.16-fold higher than the control. When treated with 3.75 mg/L of cucurbitacin B for 24 h,the I₅₀ values of dichlorvos and methomyl to the CarE were 25.94- and 2.45- fold higher than the control, respectively.While when treated with 1.0 mg/mL 2-tridecanone for 24 h,the I₅₀ values of dichlorvos,DEF and dimethoate to the CarE were decreased by 89% ,83% and 90% ,respectively.