| 甲基对硫磷人工抗原的合成与鉴定 |
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| 引用本文： | 贾明宏,钱传范,韩丽君,李成文,张冰.甲基对硫磷人工抗原的合成与鉴定[J].农药学学报,2003,5(2):22-32. |
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| 作者姓名： | 贾明宏 钱传范 韩丽君 李成文 张冰 |
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| 作者单位： | 1.中国农业大学 理学院,北京 100094 |
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| 基金项目： | 国家科技攻关计划课题“设施蔬菜有害残留快速检测技术研究”(基金号 2 0 0 1BA5 0 3B- 0 3)，国家高新技术研究发展计划“863计划”(2 0 0 1AA2 4 90 62 )子课题项目 |
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| 摘 要： | 根据甲基对硫磷的结构特征,分别从甲基对硫磷的硝基一端或磷酸酯基甲氧基一端引入一定长度的间隔臂和活性基团,设计并合成了4种结构的半抗原;针对各种半抗原所带活性基团的性质,通过多种方法制备了与不同的载体蛋白偶联的人工抗原。进行动物免疫后,得到了针对甲基对硫磷的高效价抗体。
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| 关 键 词： | 酶联免疫吸附分析 甲基对硫磷 人工抗原 |
| 文章编号： | 1008-7303(2003)02-0022-11 |
Synthesis and Identification of Antigenic Conjugate against Parathion-methyl |
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| JIA Ming-hong,QIAN Chuan-fan,HAN Li-jun,LI Cheng-wen and ZHANG Bing.Synthesis and Identification of Antigenic Conjugate against Parathion-methyl[J].Chinese Journal of Pesticide Science,2003,5(2):22-32. |
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| Authors: | JIA Ming-hong QIAN Chuan-fan HAN Li-jun LI Cheng-wen and ZHANG Bing |
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| Institution: | 1.College of Science, China Agricultural University, Beijing 100094，China2.Academy of Military Medical Sciences, Beijing 100850，China |
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| Abstract: | Four kinds of hapten with the similar structure of parathion-methyl were designed and synthesized. These four haptens were coupled with different length and structural spacer arms through nitro-group or methoxyphosphate group of parathion-methyl. Then six kinds of hapten-protein conjugates were synthesized by different methods. The immune program yielded high specific and high affinity antibodies for parathion-methyl. |
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| Keywords: | ELISA parathion-methyl synthesized antigenic conjugate |
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