大孔吸附树脂纯化紫苏叶花色素苷的研究

通过比较8种大孔吸附树脂对紫苏叶花色素苷粗提物的静态吸附和解吸性能，筛选出大孔吸附树脂XAD-7，并对其动态吸附和解吸条件(如上样液浓度、酸度、盐度、洗脱溶剂及流速等)进行研究。结果表明:将紫苏叶花色素苷上柱液用稀盐酸调节pH值至3.0，调整溶液花色素苷的浓度大于6.5 mg/mL，添加不超过10%的食盐，以10 BV/h的流速上柱5 h，至XAD-7树脂吸附饱和，然后用大量的0.1% HCl洗脱至洗脱液不含糖类等杂质，将30%的乙腈溶液用稀盐酸调节pH值至3.0，以2 BV/h的流速洗脱4 h，得到的精制品中花色素苷的含量为78.18%，树脂富集倍数为2.66。 

Purification of anthocyanins from perilla leaves with macroporous adsorbing resins

By comparing static adsorption and desorption property of anthocyanins crude extract from perilla leaves with 8 macroporous adsorbing resins, XAD-7 was the most appropriate resin. The dynamic adsorption and desorption conditions, such as sample solution concentration, salt concentration, wash solvent and velocity were studied. The results indicate that the optimum conditions were that the pH value of loading solution was adjusted to 3.0, the concentration of anthocyanin was more than 6.5 mg/mL, the salt concentration was no more than 10% and the column was loaded at the flow rate of 10 BV per hour for 5 hours. After that, the column was rinsed by 0.1% HCl until the elution solution did not contain contamination such as sugar. Subsequently, the column was eluted by 30% acentonitrile (pH 3.0) at the flow rate of 2 BV per hour for 4 hours. Under this condition, the content of anthocyanins in the refined product was 78.18%, and XAD-7 resin enrichment amount were 2.66 multiple.