Ingestion and killing of Pasteurella haemolytica A1 by bovine neutrophils in vitro |
| |
Authors: | C J Czuprynski H L Hamilton E J Noel |
| |
Affiliation: | 1. Department of Respiratory Medicine, University Medical Center Utrecht, Utrecht, The Netherlands;2. Laboratory of Translational Immunology, University Medical Center Utrecht, Utrecht, The Netherlands;3. Department of Surgery, University Medical Center Utrecht, Utrecht, The Netherlands;4. Department of Anesthesiology and Critical Care, University Medical Center Utrecht, Utrecht, The Netherlands |
| |
Abstract: | In this study, various parameters affecting the ability of bovine neutrophils to ingest and kill a virulent strain of Pasteurella haemolytica A1 in vitro were examined. Ingestion of P. haemolytica was serum dependent (optimal serum concentration 10%) and was mediated principally by heat-stable opsonins, presumably antibodies, that could be removed by absorption with formalin-killed P. haemolytica. Ingested P. haemolytica were killed by neutrophils within 1-4 h incubation; the magnitude of killing being directly dependent on the number of neutrophils present. The number of viable P. haemolytica was reduced by approximately 1.5 log at bacterial concentrations of 0.01-100 P. haemolytica per neutrophil; a concomitant reduction in neutrophil viability was observed at the highest bacterial concentration (100:1). Bovine neutrophils underwent a vigorous luminol-enhanced chemiluminescence response after ingesting opsonized P. haemolytica, thus indicating that reactive oxygen intermediates were being formed that could have contributed to the intracellular killing of P. haemolytica. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|