| 石斛叶片蛋白质双向电泳技术体系的建立及优化 |
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| 引用本文： | 董婧,应奇才,王慧中.石斛叶片蛋白质双向电泳技术体系的建立及优化[J].浙江农业学报,2009,21(3):0. |
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| 作者姓名： | 董婧 应奇才 王慧中 |
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| 作者单位： | 杭州师范大学生物化学与分子生物学杭州市重点实验室，浙江杭州310036 |
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| 基金项目： | 国家自然科学基金，浙江省自然科学基金，浙江省科技计划项目，杭州市属高校重点实验室科技创新计划 |
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| 摘 要： | 以石斛叶片为材料，建立了一套适用于石斛蛋白质组分析的双向电泳体系，探讨了石斛叶片蛋白的提取、蛋白定量以及双向电泳的一些关键技术。结果表明，通过三氯乙酸(TCA)法提取、高压等电聚焦和两步平衡等方法可以获得稳定、清晰的双向电泳图谱，获得了可分辨蛋白质斑点数约613个。
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| 关 键 词： | 双向电泳 蛋白质组 石斛 |
Establishment and optimization of two-dimensional electrophoresis for proteomic analysis of dendrobium |
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| DONG Jing,YING Qi-cai,WANG Hui-zhong.Establishment and optimization of two-dimensional electrophoresis for proteomic analysis of dendrobium[J].Acta Agriculturae Zhejiangensis,2009,21(3):0. |
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| Authors: | DONG Jing YING Qi-cai WANG Hui-zhong |
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| Institution: | Key Laboratory of Molecular Biology and Biochemistry of Hangzhou， Hangzhou Normal university，Hangzhou 310036，China |
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| Abstract: | A 2-D(two-dimensional electrophoresis)protocol suitable for protein analysis in dendrobium leaf was set up．The protein extraction and protein excretion．as well as some key technologies of 2-D electrophoresis were studied．The resultsshowed that stable and clear 2D maps with about 613 protein spots could be obtained through the methods of extraction with trichloroacetic acid，isoelectric focusing under high-voltage and two-step equilibrium． |
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| Keywords: | two-dimensional electrophoresis proteome Dendrobium orchids |
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