Allelopathy assessments for the environmental biosafety of the salt-tolerant transgenic Eucalyptus camaldulensis, genotypes codA12-5B, codA 12-5C, and codA 20C |
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Authors: | Akira Kikuchi Xiang Yu Takayoshi Shimazaki Akiyoshi Kawaoka Hiroyasu Ebinuma and Kazuo N Watanabe |
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Institution: | (1) Gene Research Center and Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Ten-noudai, Tsukuba 305-8572, Japan;(2) Forestry Science Laboratory, Nippon Paper Industries Co., Ltd., Tokyo 114-0002, Japan |
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Abstract: | Allelopathy tests were conducted on salt-tolerant transgenic eucalyptus trees conferring bacterial codA gene in the designated net-house conditions under Type II use (contained use) of the Japanese law on environmental biosafety
aiming for Type I (field use) application. Three transgenic and corresponding nontransgenic genotypes were employed for four
different tests: (1) sandwich bioassay; (2) soil germination method; (3) gas chromatography (GC) for volatile substances from
the plants; and (4) high-performance liquid chromatography (HPLC) on phenolic compounds from fresh leaves, which are the primary
allelopathic substances on the species. The simple approaches, the bioassays, indicated no significant difference between
the transgenic and nongenetically modified genotypes. There was no qualitative difference between the transgenic and nontransgenic
lines by GC or HPLC. Absence of any quantitative difference was suggested by repetitive examination and subsequent analysis
of variance assessments with the chromatographic methods and bioassays. Moreover, it was also indicated that bioassays should
be the primary assessment method for allelopathy in considering the simplicity, speed, low cost, and reproducibility of these
methods. Overall, substantial equivalence was considered on the three transgenic genotypes with codA gene when compared with the nontransgenic Eucalyptus camaldulensis lines. The experiments supported the application to isolated field testing of the transgenic Eucalyptus camaldulensis genotypes as the first case and experience in Japanese regulatory approval processes Type I Use for the deliberate release
to the environment. |
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Keywords: | Allelopathy Bioassay Environmental biosafety Eucalyptus camaldulensis Salt tolerance |
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