| 气相色谱法测定水产品中氯霉素残留 |
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| 引用本文： | 赵文亚.气相色谱法测定水产品中氯霉素残留[J].水产学报,2003,27(3):278-282. |
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| 作者姓名： | 赵文亚 |
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| 作者单位： | 1. 南京农业大学食品科技学院,江苏,南京,210095
2. 江苏省水产质量检测中心,江苏,南京,210017 |
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| 基金项目： | 江苏省"十五"科技攻关资助项目(BE2001385) |
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| 摘 要： | 介绍了水产品中氯霉素残留量测定的气相色谱法。样品中的氯霉素用乙酸乙酯提取,正己烷去脂肪,过Sep-C18柱进行净化,用BSTFA-TMCS衍生后进带有微电子俘获器的气相色谱仪检测。该方法的线性范围为0.5～500μg·L-1,相关系数r=0.999,最低检测限为0.1μg·kg-1,相对标准偏差为4.3%～11.0%,向样品中分别添加1μg·kg-1、10μg·kg-1和20μg·kg-13个浓度水平的氯霉素,回收率分别为62.0%、88.2%和96.4%。方法灵敏度高,准确可靠,适合水产品中氯霉素残留量的检测。
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| 关 键 词： | 气相色谱法 测定技术 水产品 氯霉素 残留量 氯霉素 乙酸乙酯 微电子俘获器 |
| 文章编号： | 1000-0615(2003)03-0278-05 |
| 收稿时间： | 2014/3/18 0:00:00 |
| 修稿时间： | 2002年8月2日 |
Determination of chloramphenicol residues in aquatic products by gas chromatography |
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| ZHAO Wen ya.Determination of chloramphenicol residues in aquatic products by gas chromatography[J].Journal of Fisheries of China,2003,27(3):278-282. |
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| Authors: | ZHAO Wen ya |
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| Institution: | .Department of Food Science and Technology ,Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | A gas chromatography method had been established for the determination of chloramphenicol in aquat ic
products. Chloramphenicol was extracted with ethyl acetate and partitioned with n-hexane to remove lipids.
Cleanup was performed on a Sep cartridge. After the drug was derived with BSTFA- TMCS, the derivatives were
analyzed by capillary gas chromatography- electron capture detection. The linear range was from 0. 5..g..L
- 1
to
500..g..L
- 1
with r of 0. 999. The average recoveries were 62. 0%, 88. 2% and 96. 4% respectively when
samples were spiked w ith 1..g..kg- 1, 10..g..kg- 1 and 20..g..kg- 1. The detection limit was 0. 1..g..kg- 1. The
RSDs were 4. 3% - 11. 0% . The method is sensitive, accurate and suitable for the determinat ion of trace
chloramphenicol in aquatic products. |
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| Keywords: | aquatic product chloramphenicol gas chromatography |
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