| 两株对辣椒疫霉菌有拮抗作用的拮抗菌分泌蛋白的研究 |
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| 引用本文： | 戴晓燕,关桂兰. 两株对辣椒疫霉菌有拮抗作用的拮抗菌分泌蛋白的研究[J]. 中国生物防治, 1999, 15(2): 81-84 |
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| 作者姓名： | 戴晓燕 关桂兰 |
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| 作者单位： | 中国科学院新疆生物土壤沙漠所,乌鲁木齐,830011 |
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| 基金项目： | 中国科学院重点项目资助 |
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| 摘 要： | 用平板对峙生长法筛选到两株对辣椒疫霉菌具有较强拮抗作用的细菌，经鉴定为芽孢杆菌属。测定了无菌滤液的抑菌活性及两菌产生拮抗物的热稳定性。用硫酸铵沉淀法提取到对辣椒疫霉菌有拮抗作用的抗菌蛋白。发酵条件的研究表明，用Ｇ培养基、ｐＨ７、培养３２小时，ＸＡ１、ＸＦ１能产生具有较强拮抗作用的拮抗物。
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| 关 键 词： | 拮抗细菌；抗菌蛋白；辣椒疫霉菌；芽孢杆菌属 |
| 修稿时间： | :1998-08-1 |
Study on Two Strains of Antagonistic Bacteria Producing Antagonistic Proteins to Phytophthora capsici |
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| DAI Xiao-yan,GUAN Gui-lan. Study on Two Strains of Antagonistic Bacteria Producing Antagonistic Proteins to Phytophthora capsici[J]. Chinese Journal of Biological Control, 1999, 15(2): 81-84 |
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| Authors: | DAI Xiao-yan GUAN Gui-lan |
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| Abstract: | Two strains of bacteria XA1, XF1 were isolated from hundreds of rhizobacteria. They showed strong inhibitory action against Phytophthora capsici in plate test. A large amount of antifungal proteins were extracted with (NH4)2SO4.The antagonistic prodcuts were thermostable.Further experiments indicated that the optimum culture conditions for producing proteins were using a G medium of pH7 and cultivated for 32h. |
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| Keywords: | antagonistic bacteria antagonistic proteins Phytophthora capsici Bacillus spp. |
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