| 短蛸微卫星多重PCR体系建立及交配模式分析 |
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| 作者姓名： | 刘文芬 冯艳微 王卫军 陈建强 杨建敏 |
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| 作者单位： | 上海海洋大学水产科学国家级实验教学示范中心水产动物遗传育种中心上海市协同创新中心水产种质资源发掘与利用教育部重点实验室;山东省海洋资源与环境研究院山东省海洋生态修复重点实验室 |
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| 摘 要： | 本研究对实验室开发的12对短蛸(Amphioctopus fangsiao)高多态性微卫星标记进行优化组合,构建了4组三重PCR体系,对短蛸4个母本(♀1、♀2、♀3和♀4)、104个子代及19个候选父本进行亲权鉴定,分析其交配模式。结果显示,构建的4组三重PCR体系可有效鉴定短蛸的亲权关系,104个子代鉴定率为94.23%。♀1组样品鉴定出12个子代,父本数为3个;♀2组样品鉴定出40个子代,父本数为7个;♀3组样品鉴定出19个子代,父本数为2个;♀4组样品鉴定出27个子代,父本数为4个;并且♂2、♂3、♂7和♂10均与2个以上的母本发生了交配。由此分析得出,短蛸交配模式存在多雌多雄关系。研究结果为高效开展短蛸人工繁育、增养殖及放流工作提供了重要参考。
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| 关 键 词： | 短蛸 微卫星多重PCR 交配模式 |
| 收稿时间： | 2017/4/26 0:00:00 |
| 修稿时间： | 2017/5/23 0:00:00 |
Development of Microsatellite Multiplex PCR Sets and Its Application in the Reproduction Model Analysis of Amphioctopus fangsiao |
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| Authors: | LIU Wenfen FENG Yanwei WANG Weijun CHEN Jianqiang and YANG Jianmin |
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| Institution: | ational Demonstration Center for Experimental Fisheries Science Education; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education; Shanghai Ocean University, Shanghai 201306;Shandong Marine Resource and Environment Research Institute, Shandong Provincial Key Laboratory of Marine Ecological Restoration, Yantai 264006,Shandong Marine Resource and Environment Research Institute, Shandong Provincial Key Laboratory of Marine Ecological Restoration, Yantai 264006,Shandong Marine Resource and Environment Research Institute, Shandong Provincial Key Laboratory of Marine Ecological Restoration, Yantai 264006,Shandong Marine Resource and Environment Research Institute, Shandong Provincial Key Laboratory of Marine Ecological Restoration, Yantai 264006 and Shandong Marine Resource and Environment Research Institute, Shandong Provincial Key Laboratory of Marine Ecological Restoration, Yantai 264006 |
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| Abstract: | |
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| Keywords: | Amphioctopus fangsiao Microsatellite multiplex PCR Paternity |
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