中药合剂对雏鸡免疫功能的影响

研究了中药合剂对雏鸡免疫功能及干扰素产生的影响。检测了试验组和对照组28,30,34,41,48,55日龄雏鸡血液T淋巴细胞对丝裂原ConA刺激的反应性;CD4+、CD8+T淋巴细胞亚群及CD4+/CD8+T淋巴细胞比值;ND抗体效价及胸腺、脾脏和法氏囊指数。结果表明,34~41日龄时中药合剂组雏鸡血液T淋巴细胞对丝裂原ConA刺激的反应性明显高于对照组(P<0.05);CD4+、CD8+T淋巴细胞亚群无明显变化,30~34日龄时能明显提高CD4+/CD8+淋巴细胞亚群比值(P<0.05);34~41日龄时试验组雏鸡HI抗体效价显著高于对照组(P<0.05);可使胸腺、脾脏和法氏囊指数增加,41日龄时法氏囊指数明显高于对照组(P<0.05);中药合剂对IFN具有明显的诱生作用(P<0.01),与NDV诱生组水平相当;同时可明显促进NDV对IFN的诱生(P<0.01)。结果显示中药合剂具有增强机体免疫功能及诱生IFN的能力,为该中药合剂抗NDV提供了理论依据。

Effect of Chinese herbal medicine mixture on immune function in chickens

The effect of Chinese herbal medicine mixture on the immune function and interferon production of the chickens were studied. The effects were determined on 28,30,34,41,48,55 days of age chickens in experimental groups and the control group, they are the proliferation of T lymphocytes induced by ConA; CD4+, CD8+ subsets of T lymphocyte and the ratio of CD4+ / CD8+ T lymphocytes; the ND antibody titer; the indexes of the thymus, spleen and Fabricius′ bursa. The results showed that the proliferation of T lymphocytes induced by ConA in blood of the experimental groups were significantly different from that of the control group by 34~41 days of age (P < 0.05); The CD4+, CD8+ subsets of T lymphocyte had no significant difference (P > 0.05), and the ratio of CD4+ / CD8+ T lymphocyte subsets were increased obviously (P < 0.05) from 30 to 34 days of age; by 34~41 days of age ,the ND antibody titer were higher significantly than that of the control group (P < 0.05); the indexes of the thymus, spleen and Fabricius′bursa were increased, the index of Fabricius′ bursa in experimental groups was higher significantly than that in the control group on 41-day-old (P < 0.05). The Chinese herbal medicine mixture could induce the production of interferon significantly (P < 0.01) and had the same interferon-inducing ability as NDV. That could also promote the induction of interferon by NDV, the level had great significant difference (P < 0.01). The results showed that the Chinese herbal medicine mixture could enhance the immune function in chickens and increase the IFN production had in chickens obviously, which might be beneficial to regulating body immunization, the theoretical evidence was provided for the Chinese herbal medicine mixture against NDV.