| 桑枝黄酮类化合物含量的测定方法 |
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| 引用本文： | 陈亚洁,张作法,黄世荣,刘明明,时连根. 桑枝黄酮类化合物含量的测定方法[J]. 蚕桑通报, 2015, 0(3): 18-21. DOI: 10.3969/j.issn.0258-4069.2015.03.006 |
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| 作者姓名： | 陈亚洁 张作法 黄世荣 刘明明 时连根 |
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| 作者单位： | 1. 浙江大学 动物科学学院,浙江 杭州,310058;2. 永康市经济特产站,浙江 永康,321300 |
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| 基金项目： | 浙江省科技特派员团队项目，金华市科技计划项目 |
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| 摘 要： | 研究了桑枝黄酮类化合物含量的测定方法。结果将桑枝过160目,选用80%乙醇液作为提取溶剂,超声波处理20 min后在80℃下回流30 min,重复抽提3次,可以有效地提取桑枝中黄酮类化合物。以Na NO2-A1(NO3)3显色,用紫外分光光度计于510 nm波长下测定吸光度,能精确、稳定地检测出桑枝中黄酮类化合物含量。
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| 关 键 词： | 桑枝 黄酮类化合物 含量测定 |
The Determination Method of Flavonoid Contents in Ramulus mori |
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| Abstract: | The determination method of flavonoid contents in Ramulus mori was studied in this article. The flavonoid could be effectively extracted from Ramulus mori after treated with the process: it was sifted with 160 item, use the sol-vent of 80% ethanol, extracted with the ultrasonic for 20 minutes and the circumfluence for 30 minutes at 80 ℃ for three times. Using NaNO2-Al(NO3)3 as color reaction reagent, the flavonoid contents could be exactly and stably detected with ultraviolet spectrophotometer under wavelength of 510 nm. |
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| Keywords: | Ramulus mori flavonoid compounds content determination |
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