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茎枯病菌毒素制备及对芦笋愈伤组织抗性诱导的研究
引用本文:李俊萍,乜兰春,王珊珊,刘孟.茎枯病菌毒素制备及对芦笋愈伤组织抗性诱导的研究[J].植物病理学报,2016,46(1):140-144.
作者姓名:李俊萍  乜兰春  王珊珊  刘孟
作者单位:河北农业大学 园艺学院,保定 071000
基金项目:河北省现代农业产业技术体系蔬菜创新团队
摘    要:<正>芦笋(Asparagus officinalis L.)又名石刁柏,是百合科天门冬属多年生宿根植物,是世界公认的高级保健蔬菜。我国是芦笋第一大生产和出口大国,但茎枯病的危害严重影响了芦笋的产量与质量。芦笋茎枯病是由天门冬拟茎点霉菌(Phmopsis asparagi(Sass.)Bubak)引起的一种真菌性病害,因气候原因,我国3年以上笋田发病率几乎100%,而


Phomopsis asparagi toxin preparation and the resistance induction on asparagus callus by the pathogen toxin
LI Jun-ping,NIE Lan-chun,WANG Shan-shan,LIU Meng.Phomopsis asparagi toxin preparation and the resistance induction on asparagus callus by the pathogen toxin[J].Acta Phytopathologica Sinica,2016,46(1):140-144.
Authors:LI Jun-ping  NIE Lan-chun  WANG Shan-shan  LIU Meng
Institution:College of Horticulture, Agricultural University of Hebei, Baoding 071000, China
Abstract:Toxin-producing culture medium and culture time for Phomopsis asparagi were optimized, and the effect of the pathogen toxin on asparagus callus was investigated in the study. It was found that the toxin produced from culture for 12 days in the Fries medium showed the strongest inhibition effect on asparagus radicle. Toxin caused callus of ‘Apollo’, ‘Champion’ and ‘NJ987’ browning. Resistance of the callus of the three varieties to toxin could be induced by toxin treatment. Toxin concentration for resistant callus selection was 30%-40%.
Keywords:Phomopsis asparagi  toxin  radicle inhibition rate  callus  browning  
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