| 山羊核移植胚胎干细胞的分离培养 |
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| 引用本文： | 葛秀国,李吉霞,窦忠英. 山羊核移植胚胎干细胞的分离培养[J]. 安徽农业科学, 2011, 39(7): 4012-4014 |
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| 作者姓名： | 葛秀国 李吉霞 窦忠英 |
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| 作者单位： | 1. 天津农学院动物科学系,天津,300384
2. 西北农林科技大学陕西省干细胞工程技术研究中心,陕西,杨凌,712100 |
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| 基金项目： | 国家高技术研究与发展项目 |
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| 摘 要： | 比较了不同发育阶段山羊核移植胚胎分离培养胚胎干细胞（NT-ESCs）的情况。结果表明,各阶段山羊核移植胚胎均可分离出NT-ESCs,其中孵化胚的NT-ESCs克隆形成率最高（38%）,与囊胚（26%）差异显著,与桑葚胚（18%）差异极显著。所分离的NT-ESCs细胞形态与正常ESCs相似,均具有碱性磷酸酶活性,可表达多能性基因Oct-4和表面标志抗原SSEA-1。
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| 关 键 词： | 山羊 核移植 胚胎干细胞 |
Isolated Culture of Nuclear Transplantation Embryonic Stem Cells of Goat |
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| Affiliation: | GE Xiu-guo et al(Department of Animal Science,Tianjin College of Agronomy,Tianjin 300384) |
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| Abstract: | The isolated cultures of nuclear transplantation embryonic stem cells(NT-ESCs) from nuclear transplantation embryos of goats in different developmental stages were compared.The results showed that NT-ESCs could be isolated from nuclear transplantation embryos of goats in all developmental stages,among which the cloning efficiency of NT-ESCs from hatching embryos was highest,being 38%;it had significant difference from that of blastula,which was 26% and it had extremely significant difference from that of embryotic sphere,which was 18%.The shape of isolated NT-ESCs was similar to that of normal ESCs,both of them had alkaline phosphatase activities and could express pluripotency gene Oct-4 and surface marker antigen SSEA-1. |
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| Keywords: | Goat Nuclear transplantation Embryonic stem cells |
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