| 改良CTAB法提取鸢尾属植物DNA |
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| 引用本文： | 李承哲,刘占林,杨红超,黄安玲,刘芳.改良CTAB法提取鸢尾属植物DNA[J].现代农业科技,2011(8):31+34. |
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| 作者姓名： | 李承哲 刘占林 杨红超 黄安玲 刘芳 |
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| 作者单位： | 西北大学生命科学学院,陕西西安,710069 |
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| 摘 要： | 鸢尾属植物中酚类、多糖含量较高,DNA提取较为困难。在前人工作基础上,改进了提取植物DNA的CTAB方法,通过延长水浴时间、氯仿/异戊醇(24∶1)2次抽提、适当延长氯仿/异戊醇与提取液接触时间等一系列改进,简化了提取步骤,大大缩短了提取时间。所提取的DNA纯度高,可满足以PCR扩增为基础的试验需要。
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| 关 键 词： | 鸢尾属 DNA提取 改良CTAB法 PCR |
DNA Extraction from Iris Species with Modified CTAB Method |
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| Authors: | LI Cheng-zhe LIU Zhan-lin YANG Hong-chao HUANG An-ling LIU Fang |
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| Institution: | LI Cheng-zhe LIU Zhan-lin * YANG Hong-chao HUANG An-ling LIU Fang |
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| Abstract: | DNA isolation of Iris species is difficult for their high contents of polyphenol and polysaccharide.Here a modified CTAB method for Iris DNA extraction was presented.With series of improvement and simplification of the procedure,including water-bath time extension,chloroform/ isoamyl alcohol(24∶1) twice extraction,high concentration and purity of DNA was obtained and it could be used in the following molecular biology experiments based on PCR amplification. |
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| Keywords: | PCR |
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