小豆SSR引物在绿豆基因组中的通用性分析

分析了187对小豆SSR引物在绿豆基因组中的可转移性,以期为绿豆分子遗传育种研究提供分析工具.结果表明,约75%的小豆SSR引物可在绿豆中有效扩增,但不同小豆连锁群SSR引物的可转移率存在差异.多态性分析发现80对引物中有28对在60份绿豆种质中可以检测到多态性,等位变异数从2～7不等,平均为2.9,PIC指数从0.02～0.69,平均为0.36.UPGMA聚类及主坐标分析表明,尽管同一省份的种质不能紧密聚在一起,但大多在聚类图上成簇状分布,说明相同来源的绿豆种质具有相似的遗传背景;此外,国外及我国边远地区的绿豆种质在遗传背景上与内部省份间存在明显差异.这些多态性SSR不仅可以有效用于绿豆分子遗传学研究,还可以用于不同来源绿豆种质资源的辅助鉴别.

Transferability of SSR from Adzuki Bean to Mungbean

SSR (Simple sequence repeat) has been a popular tool in genetic studies. However, only a few SSR markers are available in mungbean (Vigna radiata L.). Adzuki bean (Vigna angularis L.) is a close relative of mungbean. In the study reported in this paper, we tested the transferability of adzuki bean SSR markers into mungbean. A total of 187 sets of SSR primers were tested and about 75% of them generated repeatable and clear products in mungbean, indicating a high homology between these two genomes. Of the transferable SSRs, 28 sets of primers generated polymorphic products among 60 mungbean genotypes with a total of 81 alleles detected. The average PIC (polymorphic information content) value is 0.36. Based on the SSR fingerprints, the 60 accessions can be divided into different groups agreeing well with their geographical origins. The exotic accessions were well separated from the local genotypes, indicating the former could be important sources for broadening the mungbean gene pool. The present study identified useful SSR markers for mungbean research, which will undoubtedly accelerate the genetic study of this crop.