少量油菜种子含油量快速测定方法及应用研究

构建了一种高通量的、测定少量油菜种子（约200mg）中粗脂肪含量的超声波提取装置，通过对种子浸泡时间、超声波提取时间、超声波提取温度做三因素三水平正交试验确定了最佳提取条件为：浸泡时间1.5h，超声波提取时间60min，超声波提取温度50℃。在优化的条件下，该方法测得同品种油菜籽样品中粗脂肪含量的相对标准偏差(RSD)小于3.6%（n＝8），具有较好的重现性；且测定结果与标准方法相一致，具有较高的准确性。实验结果表明，本研究所构建的超声波提取法可满足一般少量油菜种子中粗脂肪含量的准确测定。该方法的建立，为油菜育种和基因工程研究中少量样品含油量的测定提供了一种快速测定的方法。

A quick method for oil content determination in small quantity of rape seed

A simple, low cost and effective solvent extraction method was developed using ultrasonic for oil content determination in small quantity (about 200mg) of rape seed. Oil extraction efficiency was investigated through an orthogonal experiment (L_9 (3~4)) using ultrasound-assisted extraction. Statistical analysis showed an optimal extraction was under an immerging time in petroleum ether for 1.5 h,an ultrasound-assisted extraction time for 60 min and an extraction temperature of 50℃.With the new method,the oil contents in small quantity of seed were successfully determined and the results were consistent with that of traditional standard method.