Collembola effects on plant mass and nitrogen acquisition by ash seedlings (Fraxinus pennsylvanica)

We studied the effects of varied collembolan numbers on three compensatory mechanisms of nutrient uptake: fine root mass, endomycorrhizal development, and physiological uptake capacity. We grew ash (Fraxinus pennsylvanica) with or without the arbuscular mycorrhizal fungus Glomusintraradices, with 0, 10 or 50 initial Collembola (Folsomia candida). After 83 d root and uptake rates, endomycorrhizal development, and plant biomass were determined. Plant mass increased with Collembola number. Collembola interacted with mycorrhizae in their effects on N uptake and leaf N. Collembola in the absence of mycorrhizal roots were associated with lower N uptake and leaf N at 10 than at 0 or 50 initial Collembola. In contrast, Collembola in the presence of mycorrhizal roots were associated with the highest rate of N uptake and leaf N at 10 versus 0 or 50 initial Collembola. Hence as initial Collembola number increased, the relative importance of root system traits that determined N uptake changed from root physiological uptake capacity, presence of mycorrhizal roots, to fine root biomass.