A型副鸡嗜血杆菌(山东株)的分离与鉴定

2011年11月,山东某肉鸡场发生以肉鸡眼睑及眶下窦周围明显肿胀为主要特征的传染性疾病。从采集的发病鸡眶下窦中分离到1株细菌,根据发病鸡群的临床症状、细菌的分离培养、形态观察、过氧化氢酶试验、革兰氏染色、动物回归试验、免疫攻毒试验和PCR等方法初步鉴定为副鸡嗜血杆菌(Hpg)。此外通过分离菌株的水平传播试验结果表明,造成该鸡场Hpg的水平传播和鸡传染性鼻炎的反复发作与饲养密度有很大的关系。最后将分离菌株送匈牙利诗华研发中心进行分型鉴定,确定为A型副鸡嗜血杆菌。根据分离地点将分离菌株命名为A型副鸡嗜血杆菌(山东株)。另外通过与GenBank已发表的Hpg基因序列进行同源性比对分析,结果显示,该基因序列与参考株的基因核苷酸序列同源性达99.0%。

Isolation and Identification of Haemophilus paragallinarumSerotype A in Shandong Province

In November 2011, an avian disease was occurred in a local chicken farm in Shandong province characterized by obviously swell in and around the eyelid and the subocular sinus. One bacterial strain was isolated from the infected chickens, and the bacterial strain was initially identified as Haemophilus paragallinarum (Hpg) based on the following methods,clinical symptoms of the infected chicken, isolation and culture of bacterial strain,morphological observation, catalase test, gram stain, animal regression test,vaccination and bacterial challenge experiments and PCR. In addition, the horizontal transmission test of the isolated bacterial strain suggested that the horizontal transmission of Hpg and repeated outbreak of IC in this farm were much related to the stocking density. Finally,the isolated bacterial strain was sent to R&D center of CEVA in Hungary for further identification, and the strain obtained from the chicken farm in Shandong province was serotype A Hpg(Shangdong strain).Besides,through the homologous comparison and analysis with the Genbank published Hpg gene sequence, it indicateed that homology of nucleotide sequence between our isolated strain and the reference strain could reach 99.0%.