多花黑麦草品种间杂交F2代分子标记遗传分析

运用序列相关扩增多态性(SRAP)和微卫星标记(SSR)技术对6个多花黑麦草亲本品种及其9个杂交F₂代组合80份材料进行遗传分析。筛选出具有多态性的15对SRAP引物、16对SSR引物,SRAP标记平均每对引物扩增出16.7条带,多态性位点百分率为68.88％。SSR标记平均每对引物扩增出14.75条带,多态性位点百分率为58.05％。通过聚类树状图分析得出结论,杂交F₂代内同一组合基本聚为一类,长江2号×剑宝和长江2号×阿伯德组合除外;父本和母本与其杂交F₂代遗传距离与聚类结果基本一致。同时还发现SRAP标记对杂交F₂代及亲代聚类分析可靠性高于SSR标记。

A study on genetic analysis of F2 hybird progenies by molecular markers in annual ryegrass (Lolium multiflorum)

Six parental cultivars of Lolium multiflorum and eighty F2 progenies of nine cross combinations were used in this study.SRAP and SSR markers were used to analyze genetic relationship of 86 L.multiflorum accessions.15 and 16 primer pairs were selected by SRAP and SSR,respectively.16.7 bands were amplified per primer pairs on average and percent of polymorphic bands was 68.88% in SRAP analysis.The average number of bands per primer pairs was 14.75 and percent of polymorphic bands was 58.05% by SSR markers.The conclusion of analyze by clustering tree showed that progenies from the same F2 cross combination clustered together,except the combination Changjiang No.2×Jumbo,Changjiang No.2×Aubade;the genetic distance from F2 progenies to male parent or female parent was so approximate.Meanwhile,the reliability of UPGMA dendrogram for L.multiflorum F2 progenies and parental cultivars by SRAP markers was higher than SSR.