Identification of parvovirus in the bone marrow of eight cats |
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| Authors: | Haynes S M Holloway S A |
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| Affiliation: | Small Animal Medicine Department, University of Melbourne, 250 Princes Hwy, Werribee, Victoria 3030, Australia. soph_haynes@hotmail.com |
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| Abstract: | Objective To determine if canine parvovirus (CPV) or feline panleucopenia virus (FPV) genomic sequences are present in adult feline bone marrow samples. Design Bone marrow samples were obtained from 32 semi‐feral cats that were euthanased at an animal shelter. DNA was extracted and subjected to conventional polymerase chain reaction (PCR) designed to determine if CPV or FPV DNA was present. Positive PCR products were purified, cloned and sequenced to differentiate between CPV and FPV. Results Eight of the bone marrow samples contained parvoviral DNA (7 CPV, 1 FPV). Conclusion CPV and FPV DNA can be found in the bone marrow of healthy adult cats. |
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| Keywords: | bone marrow canine parvovirus cats feline panleucopenia virus polymerase chain reaction |
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