健康中国恒河猴各淋巴组织中T淋巴细胞表型及分布

试验旨在明确T淋巴细胞在中国恒河猴各组织中的表型与分布,为疾病模型研究提供基础数据。取外周血、腹股沟淋巴结、肠系膜淋巴结及肠道组织,从中分离出淋巴细胞。使用流式细胞术检测分析各种表型的淋巴细胞在组织间的分布。结果表明,淋巴结中CD4⁺ /CD8⁺ T细胞比值高于外周血,肠道固有层中最低,三者差异显著。记忆性T细胞在外周血和肠道固有层T细胞中比重较大,而淋巴结中主要为幼稚T细胞。CD4⁺ T细胞中中心记忆T细胞Tcm为主要亚群,而CD8⁺ T细胞主要为效应记忆细胞Tem。外周血与肠道固有层中增殖T细胞比例相当,而淋巴结中T细胞增殖水平相对较低。各组织中CXCR4受体表达量普遍高于CCR5受体,其中肠道固有层CCR5受体表达水平最高。值得注意的是,有一小群表型CD3⁺ CD4⁺ CD8low的细胞仅在肠道固有层中存在,经分析其功能活性应高于肠道CD4单阳性T细胞。因此,测定了健康中国恒河猴各表型T淋巴细胞在多种淋巴组织中的基础数值,为相关模型研究奠定基础。

Phenotype and Distribution of the Lymphocyte in a Variety of Lymphoid Tissue in Healthy Chinese-origin Rhesus Macaques

To quantitate and phenotype the T lymphocytes in a variety of lymphoid tissue in healthy Chinese-origin rhesus macaques.Lymphocyte composition of peripheral blood(PBL),inguinal lymph nodes(ILN),mesenteric lymph nodes(MLN),intestinal lamina propria(LP) from healthy rhesus monkeys was characterized in detail by flow cytometry.The results showed that CD4+ /CD8+ ratio in LN showed higher than that in the PBL and lowest in LP,there were significant differences among them.In the LN,most CD4+ T cells were naive T cells while in the PBL and LP most CD4+ T cells were memory T cells.In all lymphoid tissues,memory markers were Tcm from the vast majority of CD4+ T cells,but most of the CD8+ T cells expressed a memory phenotype of Tem.The population of proliferating CD4+ and CD8+ T cell was similar from both PBL and LP,and higher than that from LN.The chemokine receptors CXCR4 were expressed higher than CCR5 in all lymphoid tissues.28% of CD4+ T cells in LP expressing the CCR5 that was the highest among all tissues.It was notable that there is small population of CD3+ CD4+ CD8low cells exist only in LP.The results provides a basic reference values for further research related to immunity or AIDS model by using macaques.