番茄Cf-4-Avr4互作系统中信号转导基因的克隆与功能分析

 通过基因序列分析从抗叶霉病的番茄(含Cf-4基因) 中筛选出1个参与植物过敏反应的基因RGL (Resistance Gene L ike) 。采用酵母双杂交方法, 从番茄cDNA文库中筛选出两个与该基因的编码蛋白发生互作的蛋白, 分别命名为RGLIP-1和RGLIP-2 (RGL Interacting Protein) 。RGLIP-1全长291个氨基酸, 与类囊体内腔蛋白同源性较高, RGLIP-2全长248个氨基酸, 与拟南芥转导素高度同源。利用病毒诱导的基因沉默(Virus-Induced Gene Silencing, VIGS) 技术进行了基因功能分析。结果表明, 这两个互作蛋白参与了与植物抗病相关的过敏反应(Hypersensitive Response, HR) 。

Cloning and Functional Analysis of the Genes Involved in Signal Transduction in Tomato Cf-4-Avr4 Pathosystem

The Hypersensitive Response(HR) is one of the most efficient and common disease resistance mechanisms in plant.Cloning signaling genes is very important to elucidate the resistance mechanisms.A gene in tomato with homology to several resistance proteins involved in HR in plant,was indentified and designated as RGL(Resistance Gene Like).RGL protein was used as bait to screen interacting protein(s) from tomato cDNA library through yeast two-hybrid system.Two interacting proteins designated as RGLIP-1 and RGLIP-2(RGL Interacting Protein) respectively were found.RGLIP-1 is a protein of 291 amino acids with significant homology to thylakoid lumen protein.RGLIP-2 is a protein of 248 amino acids with significant homology to transducin protein.Virus-Induced Gene Silencing(VIGS) of the two genes resulted in a partial and complete suppression of Avr4-induced HR,indicating that both genes are involved in hypersensitive response.