猕猴桃良种‘杨氏金红50号’离体再生技术

为实现‘杨氏金红50号’猕猴桃组织培养工厂化育苗，以其组培苗的叶片、叶柄为外植体直接诱导不定芽，研究不同植物生长调节剂对不定芽诱导、生根诱导的影响，筛选出高效离体再生方式。结果表明：直接诱导叶片产生不定芽的最佳培养基为MS+2 mg/L 6-BA+0.2 mg/L NAA，平均褐化率为7.5%，平均发芽率为92.5%；直接诱导叶柄产生不定芽的最佳培养基为MS+2 mg/L 6-BA+0.3 mg/L NAA，平均发芽率为89.17%；生根培养的最佳培养基为1/2 MS+0.9 mg/L IBA，根扎在培养基内部，平均愈伤组织块直径为1.03 cm，平均生根率为100%，平均根长为3.19 cm，平均生根数量为12.58条，最适开盖炼苗时间为7 d。

In Vitro Regeneration Technology for Actinidia chinensis ‘YangshiJinhong50’

In order to achieve factory seedling of Actinidia chinensis ‘YangshiJinhong 50’ by tissue culture, the leaves and petioles of tissue culture seedlings of Actinidia chinensis ‘YangshiJinhong 50’ were used as explants to directly induce adventitious buds. The effects of different plant growth regulators on adventitious bud induction and rooting induction were studied and efficient methods of in vitro regeneration were screened. The results show that the optimal medium for direct induction of adventitious buds was MS+2 mg/L 6-BA+0.2 mg/L NAA. The average browning rate was 7.5%, and the average germination rate was 92.5%. The optimal medium for directly inducing adventitious buds from petiole was MS+2 mg/L 6-BA+0.3 mg/L NAA, and the average germination rate was 89.17%. The best medium for rooting culture was 1/2 MS+0.9 mg/L IBA. The roots were shoved in the medium. The average size of callus was 1.03 cm. The average rooting rate was 100%, and the average root length was 3.19 cm. The average number of roots was 12.58, and the optimum time for hardening-seedling was 7 d.