复合酶法提取海黍子中海藻酸钠的工艺研究

海黍子是北太平洋西部特有的暖温带性褐藻,主要分布在我国黄海渤海沿岸,具有较高的经济价值和生态价值,可以作为海藻酸提取的原材料。采用复合酶法(包括纤维素酶、果胶酶和木瓜蛋白酶)提取海黍子中海藻酸钠,在酶添加量、酶解温度、pH、提取时间的单因素实验基础上,通过正交实验进行优化。最佳提取工艺筛选结果为:纤维素酶添加量3%、果胶酶添加量3%、木瓜蛋白酶添加量1%、料液比1∶20,在55℃和pH 4的条件下酶解105 min,通过消化、脱色、钙析、酸化和醇沉等工艺制备海藻酸钠,提取率达到16.82%。结果为海黍子的高值化加工提供有效的技术途径。

Study on Extraction Process of Sodium Alginate in Sargassum kjellmaniamum by Complex Enzymes Method

Sargassum kjellmaniamum is a warm temperate brown algae specially located in western North Pacific Ocean and mainly distributed along the Yellow Sea and Bohai Sea coast of China. It is of higher economic and ecological value and can be used as raw material for extracting marine algae. This study extracted sodium alginate from S. kjellmanianum by compound enzymes method (cellulase, pectinase and papain). On the basis of single factor experiment, including enzyme adding quantity, extracting temperature, pH, extract time, the orthogonal experiment was conducted to optimize the extraction process. Results showed that the optimal extraction technology including adding 3% cellulase, 3% pectinase, 1% papain, the solid to liquid ratio was 1∶20, 55℃ and pH 4 for 105 min. Sodium alginate was manufactured through digestion, decoloration, calcium analysis, acidification, and alcohol precipitation, etc. technology. The extraction rate of sodium alginate could reach 16.82%. These results provided effective technological approaches for high value processing of S. kjellmaniamum.