The transmission by nematodes of tobraviruses is not determined exclusively by the virus coat protein |
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| Authors: | Stuart A MacFarlane Derek J F Brown John F Bol |
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| Institution: | (1) Scottish Crop Research Institute, Invergowrie, DD2 5DA Dundee, Scotland, UK;(2) Gorlaeus Laboratories, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands |
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| Abstract: | The coat protein gene of the nematode non-transmissible, SP5 isolate of pea early-browning tobravius was replaced with that of the highly nematode transmissible, PPK20 isolate of tobacco rattle tobravirus. Plants were infected with the recombinant virus when mechanically inoculated and the virus invaded the plants systemically. However, although the PPK20 isolate of TRV was transmitted by nematodes from these plants, the recombinant virus was not transmitted. Therefore, the virus coat protein is not the exclusive determinant of nematode transmission. |
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| Keywords: | Pea early-browning virus recombination tobacco rattle virus Trichodorus Paratrichodorus |
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