Inhibition of Df-protease associated with allergic diseases by polyphenol. |
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| Authors: | Y Noguchi K Fukuda A Matsushima D Haishi M Hiroto Y Kodera H Nishimura Y Inada |
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| Institution: | Toin Human Science and Technology Center, Department of Material Science and Technology, Toin University of Yokohama, Aoba-ku, Japan. |
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| Abstract: | It was reported that Df-protease from house dust mite (Dermatophagoides farinae) catalyzes the activation of the kallikrein-kinin system in human plasma and is closely associated with mite-induced allergy. Therefore, to prevent the release of kinin by Df-protease, the inhibitory activity of polyphenols including catechins and flavonols was tested in vitro and in vivo. Among them, myricetin and epigallocatechin gallate (EGCg) effectively inhibited the amidase activity of Df-protease with K(i) values of 1 x 10(-)(8) and 6 x 10(-)(4) M, respectively. The kinin release in human plasma was extensively inhibited by the addition of EGCg in comparison with myricetin. Enhancement of vascular permeability in guinea pigs caused by Df-protease was markedly suppressed by EGCg. |
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