羚牛骨髓间充质干细胞的分离培养与鉴定

分离鉴定羚牛骨髓间充质干细胞，为开展羚牛细胞治疗与体细胞核移植的深入研究提供有效载体。分离培养来源于羚牛骨髓的间充质干细胞；通过染色体G显带技术进行核型分析；利用流式细胞技术检测细胞表面标记物；通过定向诱导培养探究细胞的分化潜能。结果表明，羚牛骨髓间充质干细胞能够维持正常核型，对间充质干细胞特异性表面标记 CD105、CD73、CD90、CD166、CD44呈阳性，对 CD14(单核细胞和巨噬细胞标记物)，CD19(b细胞标记物)，CD34(造血干细胞标记物)，CD45(泛白细胞标记物)和 HLA-DR均呈阴性，具有成脂、成骨与成软骨多向分化潜能。表明成功分离羚牛骨髓间充质干细胞并对其表面抗原与分化潜能进行鉴定，为羚牛生物多样性保护与内源物种间充质干细胞的应用研究奠定基础。

Isolation and Identification of Mesenchymal Stem Cells from Bone Marrow of Takin(Budorcas taxicolor)

We isolated mesenchymal stem cells(MSCs) from takin bone marrow and cultivated them in vitro to provide effective vectors for the further study of cell therapy and somatic cell nuclear transplantation of takins. The karyotypes were analyzed by the chromosome G-banding technique. Flow cytometry was also used to detect specific surface antigens. The differentiation potential of the cells was explored by directional induction culture. The results showed that cells with a normal karyotype were positive for the MSC markers CD105,CD73,CD90,CD166 and CD44,but negative for CD14(monocyte and macrophage marker),CD19(B cell marker),CD34(hematopoietic stem cell marker),CD45(pan-leukocyte marker) and HLA-DR. The cells had multidirectional differentiation potential of adipogenesis,osteogenesis and chondrogenesis. In this study,MSCs were successfully isolated from takins,and their surface antigens and differentiation potential were identified. This study laid a foundation for the conservation of takin biodiversity and the application of mesenchymal stem cells from endogenous species.