醋栗优良品种“坠玉”组织培养技术研究

以"坠玉"幼嫩茎段为外植体,研究组织培养技术,结果表明:外植体消毒最佳方法为0.1%升汞4~6 min+20%双氧水4~6 min,无菌率为39.8%;无菌外植体分化率达40%。最佳增殖培养基为MS(2倍铁盐)+6-BA0.5~0.8 mg·L-1+IBA0.2~0.5 mg·L-1,增殖倍数达5~6倍;最佳生根培养基为1/2MS+IBA0.1 mg·L-1+NAA0.1 mg·L-1,生根率达90%以上,生根数6条以上。

The tissue culture technology research of fine varieties "Zhuiyu" of Ribes nigrum

The tissue culture technology was researched by choosing tender stem of "Zhuiyu"as explant.The resulted showed that the best method of explant sterilization was 0.1%mercury bichloride 4~6min+20%hydrogen peroxide 4~6min.The sterility rate was 39.8%.Sterility explant differentiation rate was 40%.The best enrichment medium was MS(2 times molysite)+6-BA0.5~0.8 mg· L-1 +IBA0.2~0.5 mg· L-1 .The proliferation times was 5~6 times.The best root media was 1/2MS+IBA0.1 mg· L-1 +NAA0.1 mg· L-1 .The rooting percentage was more than 90%and the roo-ting amount was more than 6.