黄粉虫抗菌肽的生物学性质及初步分离纯化

［目的］探索黄粉虫抗菌肽的生物学性质。［方法］通过菌液注射对黄粉虫幼虫进行抗菌肽的诱导，对其热敏感性、反复冻融的稳定性、抑菌活性的适宜pH值及部分抑菌谱进行测定，并对黄粉虫血淋巴抗菌肽进行了初步的分离纯化。［结果］黄粉虫抗菌肽热稳定性好，反复冻融对其活性影响不大，显示出一定的抗酸碱性能，在pH为5时抑菌效果最佳，对测试的6种动物病原细菌均有不同程度的抑制作用，特别是对猪致病性大肠杆菌和猪链球菌，具有较强的抑菌活性。经SephadexG-25凝胶过滤柱层析分离得到3个活性峰，表明黄粉幼虫血淋巴中有多个抗菌活性组分。［结论］该研究为黄粉虫抗菌肽的利用奠定了理论基础。

Biological Properties and Preliminary Purification of Antibacterial Peptide from Tenebrio molitor

Objective] The aim was to explore the biological properties of antibacterial peptide from Tenebrio molitor.Method] The antibacterial peptide was induced from larvae of T.molitor by injecting bacteria solution and then they were determined in heat sensitivity,stability by repeated freezing and thawing,suitable pH for antibacterial activity and partial antibacterial spectra,and the antibacterial peptide from T.molitor haemolymph were preliminary isolated and purified.Result] The antibacterial peptide from ...