Genetic immunization with the immunodominant antigen P48 of Mycoplasma agalactiae stimulates a mixed adaptive immune response in BALBc mice |
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| Authors: | Bernardo Chessa, Marco Pittau, Maria Puricelli, Rosanna Zobba, Elisabetta Coradduzza, Paola Dall&#x Ara, Sergio Rosati, Giorgio Poli,Alberto Alberti |
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| Affiliation: | aSezione di Malattie Infettive del Dipartimento di Patologia Speciale e Clinica Veterinaria, Università degli Studi di Sassari, Via Vienna 2, 07100 Sassari, Italy;bDipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Università degli Studi di Milano, Milano, Italy;cDipartimento di Produzioni Animali, Epidemiologia ed Ecologia, Università degli Studi di Torino, Torino, Italy |
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| Abstract: | A DNA vaccine against contagious agalactia was developed for the first time, encoding the P48 of Mycoplasma agalactiae. Specific immune responses elicited in BALB/c mice were evaluated. Both total IgG and IgG1 were detected in mice vaccinated with pVAX1/P48. Proliferation of mononuclear cells of the spleen, levels of gamma interferon, interleukin-12, and interleukin-2 mRNAs were enhanced in immunized animals. Results indicate that pVAX1/P48 vaccination induced both Th1 and Th2 immune responses. Nucleic acid immunization could be a new strategy against M. agalactiae infections and may be potentially used to develop vaccines for other Mycoplasma diseases. |
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| Keywords: | Genetic immunization DNA vaccines Mycoplasma agalactiae Artificial active immunity |
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