不同激素配比对库拉索芦荟愈伤组织诱导的影响

以MS为基本培养基，添加不同浓度的2，4-D、NAA、KT或其组合，研究库拉索芦荟愈伤组织的诱导及生长情况。结果表明，两种生长素均可诱导出愈伤组织，但较高浓度的2，4-D易导致褐化，而高浓度的NAA诱导出的愈伤组织含水量较高，以两种生长素配合使用再辅以低浓度的KT效果较好，其中15mg/L的KT 4mg/L的2，4-D 0．1mg/L的KT的组合，愈伤组织诱导率达到90％，且愈伤组织生长较旺盛。

Effects of phytohormones on callus induction of A.barbadensis Mill.

MS was taken as elementary medium, stem as explant,added with various concentrations of 2,4-D,NAA,KT,studies were given to the callus induction and growth of A.barbadensis Mill. The result indicated that, two auxins all could induce callus, but high concentrated 2,4-D easily led to callus browning, and callus induced by high concentrated NAA ,which water content was high and grew slowly. Using MS medium supplemented with 15 mg/L NAA and 4 mg/L 2,4-D and 0.1 mg/L KT, the callus induction rate could reach to 90% and callus grew quickly.