| 芍药组织培养技术研究 |
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| 引用本文： | 张庆瑞,孙建洲,任凝辉,董小宇,刘志敏,翟敏. 芍药组织培养技术研究[J]. 河南农业科学, 2006, 0(4): 88-90 |
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| 作者姓名： | 张庆瑞 孙建洲 任凝辉 董小宇 刘志敏 翟敏 |
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| 作者单位： | 1. 河南农业大学林学园艺学院,河南,郑州,450002
2. 中国洛阳国家牡丹基因库,河南,洛阳,471008 |
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| 摘 要： | 以芍药休眠芽为外植体,进行组织培养试验。结果表明,外植体消毒的最佳方法是用0.1%HgCl2消毒10 min,适宜的启动培养基为1/2MS GA31 mg/L 6-BA 1 mg/L,最佳增殖培养基为1/2MS 6-BA 1 mg/L KT 0.5 mg/L。采用两步生根法,组培苗生根的适宜培养基为1/2MS IBA 1 mg/L。
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| 关 键 词： | 芍药 组织培养 培养基 |
| 文章编号： | 1004-3268(2006)04-0088-03 |
| 收稿时间： | 2005-12-10 |
| 修稿时间： | 2005-12-10 |
Tissue Culture of Paeonia lactiflora Pall |
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| ZHANG Qing-rui,SUN Jian-zhou,REN Ning-hui,DONG Xiao-yu,LIU Zhi-min,ZHAI Min. Tissue Culture of Paeonia lactiflora Pall[J]. Journal of Henan Agricultural Sciences, 2006, 0(4): 88-90 |
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| Authors: | ZHANG Qing-rui SUN Jian-zhou REN Ning-hui DONG Xiao-yu LIU Zhi-min ZHAI Min |
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| Affiliation: | 1. College of Forestry and Horticulture, Henan Agricultural University, Zhengzhou 450002, China; 2. China National Peony Gene Bank, Luoyang 471008, China |
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| Abstract: | The dormant buds of Paeonia lactiflora Pall were used as the material for tissue culture in order to improve the propagation rate.It was found in this experiment that the best sterilizing procedure was for the explants to be soaked in 0.1% aqueous mercuric chloride for 10 min,the optimal cultural medium for bud induction was 1/2MS GA_3 1 mg/L 6-BA 1 mg/L,the suitable cultural medium for multiplication was 1/2MS 6-BA 1 mg/L KT 0.5 mg/L,and the preferable root-inducing medium was 1/2MS IBA 1 mg/L. |
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| Keywords: | Paeonia lactiflora Pall Tissue culture Medium |
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