| 羊栖菜和裙带菜中抗凝血活性物质的初步筛选 |
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| 引用本文： | 刘承初.羊栖菜和裙带菜中抗凝血活性物质的初步筛选[J].水产学报,2004,28(4):473-476. |
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| 作者姓名： | 刘承初 |
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| 作者单位： | 上海水产大学食品学院,上海,200090 |
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| 基金项目： | 上海水产大学校长专项基金(SFU200103) |
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| 摘 要： | Components with blood-anticoagulant activity were fractionated from marine algae Sargassumfusiforrne and Undaria pinnatifida and the activity of the components was investigated by using the method of bioassay. It was found that the 80% ethanol soluble fraction of Sargassumfusiforme exhibited no blood-anticoagulant activity, while the components obtained from the 80 % ethanol insoluble fraction (hot water extract) gave obvious bloodanticoagulant activity. A further study suggests that the principal component in the hot water extract from Sargassumfusiforrne with high blood-anticoagulant activity should be sulfated polysaccharides, in which the activity was positively correlated with the content of total sugar and fucose in sulfated polysaccharides. As for Undaria pinnatifida, the n-butanol extract fractionated from the 80% ethanol soluble fraction had blood anticoagulant activity, while the petroleum ether, ether, ethyl acetate, or water extract showed no bloodanticoagulant activity.
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| 关 键 词： | 羊栖菜 裙带菜 抗凝血活性物质 筛选 系统分离 活性追踪 硫酸多糖 岩藻糖 |
| 文章编号： | 1000-0615(2004)04-0473-04 |
| 收稿时间： | 2014/3/14 0:00:00 |
| 修稿时间： | 2002年12月13 |
Screening of the components with blood-anticoagulant activity from marine algae Sargassum fusiforme and Undaria pinnatifida |
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| LIU Cheng-chu.Screening of the components with blood-anticoagulant activity from marine algae Sargassum fusiforme and Undaria pinnatifida[J].Journal of Fisheries of China,2004,28(4):473-476. |
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| Authors: | LIU Cheng-chu |
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| Institution: | College of Food Science, Shanghai Fisheries University, Shanghai 200090, China |
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| Abstract: | Components with blood-anticoagulant activity were fractionated from marine algae Sargassumfusiforme and Undaria pinnatifida and the activity of the components was investigated by using the method of bioassay. It was found that the 80% ethanol soluble fraction of Sargassum fusiforme exhibited no blood-anticoagulant activity, while the components obtained from the 80% ethanol insoluble fraction (hot water extract) gave obvious bloodanticoagulant activity. A further study suggests that the principal component in the hot water extract from Sargassum fusiforme with high blood-anticoagulant activity should be sulfated polysaccharides, in which the activity was positively correlated with the content of total sugar and fucose in sulfated polysaccharides. As for Undaria pinnatifida, the n-butanol extract fractionated from the 80% ethanol soluble fraction had blood anticoagulant activity, while the petroleum ether, ether, ethyl acetate, or water extract showed no bloodanticoagulant activity. |
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| Keywords: | Sargassum fusiforme Undaria pinnatifida blood-anticoagulant activity sulfated polysaccharides fucose |
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